Open Access Original article

X-ray and neutron investigation of self-assembled lipid layers on a titanium surface

Maksym Golub1*, Dieter Lott1, Erik B Watkins2, Vasyl Garamus1, Berengere Luthringer1, Michael Stoermer1, Andreas Schreyer1 and Regine Willumeit1

Author Affiliations

1 Institute of Materials Research, Helmholtz-Zentrum Geesthacht Zentrum für Material und Küstenforschung (HZG), Geesthacht, Germany

2 Institute Laue-Langevin (ILL), Grenoble, France

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Biointerphases 2013, 8:21  doi:10.1186/1559-4106-8-21

Published: 19 August 2013


Titanium is the most widely preferred metal material for bone reconstruction in orthopedics and dentistry. To improve its biological performance, various coatings can be applied. In this investigation, a biomimetic coating on a model implant surface was studied in X-ray and neutron reflectivity experiments to probe the quality of this coating, which is only few nanometers thick. Titanium was deposited on polished silicon surfaces using a magnetron sputtering technique. To improve the lipid coating’s stability, a stronger van der Waals interaction was first created between the implant surface and the biomimetic coating by adding a phosphonic acid (n-octadecylphosphonic acid – OPA) monolayer onto the surfaces. Then, three monolayers of POPE (phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-ethanolamine) were transferred using the Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) techniques. The analysis of X-ray and neutron specular reflectivity data shows that OPA molecules cover the model implant surface completely and that approximately 50% coverage of POPE can be achieved by LB and LS transfer.

Self-assembled monolayer of N-octadecylphosphonic acid; POPE coating; Titanium implants; X-ray and neutron reflectivity