Open Access Original article

Direct patterning of probe proteins on an antifouling PLL-g-dextran coating for reducing the background signal of fluorescent immunoassays

Amandine MC Egea123, Emmanuelle Trévisiol1456 and Christophe Vieu137*

Author Affiliations

1 CNRS, LAAS, 7 avenue du colonel Roche, F-31400 Toulouse, France

2 Innopsys, Parc d’Activités Activestre, 31390 Carbonne France

3 CNRS, ITAV-UMS3039, F-31106 Toulouse, France

4 Université de Toulouse; INSA, UPS, INP; LISBP, 135 Av. de Rangueil, F-31077 Toulouse, France

5 INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France

6 CNRS, UMR5504, F-31400 Toulouse France

7 Univ de Toulouse, INSA, LAAS, F-31400 Toulouse, France

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Biointerphases 2013, 8:37  doi:10.1186/1559-4106-8-37

Published: 31 December 2013

Abstract

The limit of detection of advanced immunoassays, biochips and micro/nano biodetection devices is impacted by the non-specific adsorption of target molecules at the sample surface. In this paper, we present a simple and versatile low cost method for generating active surfaces composed of antibodies arrays surrounded by an efficient anti-fouling layer, capable to decrease drastically the fluorescence background signal obtained after interaction with a solution to be analyzed. The technological process involves the direct micro-contact printing of the antibodies probe molecules on a pre-coated PLL-g-dextran thin layer obtained by contact printing using a flat PDMS stamp. Compared to other blocking strategies (ethanolamine blocking treatment, PLL-g-PEG incubation, PLL-g-dextran incubation, printing on a plasma-deposited PEO layer), our surface chemistry method is more efficient for reducing non-specific interactions responsible for a degraded signal/noise ratio.

Keywords:
Microcontact printing; Surface patterning; Antifouling coating; Immunoassays; PLL-g-dextran